Leah Bushin

Undergraduate and Graduate Student 2013-2020
After Mo Lab: Postdoctoral Fellow, SIO/UCSD (with Prof. Brad Moore)

A.B. Chemistry 2014, Ph.D. Chemistry 2020

Mo Lab Publications

Bushin, L. B.; Covington, B. C.; Clark, K. A.; Caruso, A.; Seyedsayamdost, M. R. Bicyclostreptins are Radical SAM Enzyme-modified Peptides with Unique Cyclization Motifs. Nat. Chem. Biol. 2022In Press.

Clark, K. A.; Bushin, L. B.; Seyedsayamdost, M. R.RaS-RiPPs in Streptococci and the Human Microbiome. ACS Bio. Med. Chem. Au 2022,  2, 328-339.

Rued, B. E.; Covington, B. C.; Bushin, L. B.; Szewczyk, G.; Laczkovich, I.; Seyedsayamdost, M. R.; Federle, M. J. Quorum Sensing in Streptococcus mutans Regulates Production of a Novel Class of Antimicrobial Compounds. mBio202112, e02688-20.

Bushin, L. B.; Covington, B. C.; Rued, B. E.; Federle, M. J.; Seyedsayamdost, M. R. Discovery and Biosynthesis of Streptosactin, a Sactipeptide with an Alternative Topology Encoded in Commensal Bacteria in the Human Microbiome. J. Am. Chem. Soc. 2020, 142, 16265-16275.

Isley, N. A.; Endo, Y.; Wu, Z. C.; Covington, B. C.; Bushin, L. B.; Seyedsayamdost, M. R.; Boger, D. L. Total Synthesis and Stereochemical Assignment of Streptide. J. Am. Chem. Soc. 2019, 141, 17361-17369.

Clark, K. A.; Bushin, L. B.; Seyedsayamdost, M. R. Aliphatic Ether Bond Formation Expands the Scope of Radical SAM Enzymes in Natural Product Biosynthesis.  J. Am. Chem. Soc. 2019141, 10610–10615.

Xu, F.*; Wu, Y.*; Zhang, C.; Davis, K. M.; Moon, K.; Bushin, L. B.; Seyedsayamdost, M. R. A Genetics-Free Method for High-Throughput Discovery of Cryptic Microbial Metabolites. Nat. Chem. Biol. 201915, 161-168.

Caruso, A.; Bushin, L. B.; Clark, K. A.; Martinie, R. J.; Seyedsayamdost, M. R. A Radical Approach to Enzymatic β-Thioether Bond Formation. J. Am. Chem. Soc. 2019, 141, 990-997.

Bushin, L. B.; Clark, K. A.; Pelczer, I.; Seyedsayamdost, M. R. Charting an Unexplored Streptococcal Biosynthetic Landscape Reveals a Unique Peptide Cyclization Motif. J. Am. Chem. Soc. 2018, 140, 17674-17684.

Bushin, L. B.; Seyedsayamdost, M. R. Guidelines for Determining the Structures of Radical SAM Enzyme-Catalyzed Modifications in the Biosynthesis of RiPP Natural ProductsMethods Enzymol. 2018, 606, 439-460.

Xu, F.; Nazari, B.*; Moon, K.*; Bushin, L. B.; Seyedsayamdost, M. R. Discovery of a Cryptic Antifungal Compound from Streptomyces albus J1074 Using High-Throughput Elicitor Screens. J. Am. Chem. Soc. 2017, 139, 9203-9212.

Mao, D.; Bushin, L. B.; Moon, K.; Wu, Y.; Seyedsayamdost, M. R. Discovery of scmR as a Global Regulator of Secondary Metabolism and Virulence in Burkholderia thailandensis E264. Proc. Natl. Acad. Sci. USA 2017, 114, E2920-E2928.

Metelev, M.; Arseniev, A.; Bushin, L. B.; Kuznedelov, K.; Artamonova, T. O.; Kondratenko, R.; Khodorkovskii, M.; Seyedsayamdost, M. R.; Severinov, K. Acinetodin and Klebsidin, RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae. ACS Chem. Biol. 2017, 12, 814-824.

Okada, B. K.*; Wu, Y.*; Mao, D.; Bushin, L. B.; Seyedsayamdost, M. R. Mapping the Trimethoprim-Induced Secondary Metabolome of Burkholderia thailandensis. ACS Chem. Biol. 2016, 11, 2124–2130.

Schramma, K. R.*; Bushin, L. B.*; Seyedsayamdost, M. R.  Structure and Biosynthesis of a Macrocyclic Peptide Containing an Unprecedented Lysine-to-Tryptophan Crosslink.  Nature Chem. 20157, 431-437.


NSF Graduate Research Fellowship (2017)
Hubert Alyea ’24 Teaching Award (2017)