Publications

Princeton Publications

118. Clark, K. A.; Seyedsayamdost, M. R. 2022, Submitted.

117. Covington, B. C.; Seyedsayamdost, M. R. 2022, Submitted.

116. Li, Y.; Lee, S. R.; Han, E. J.; Seyedsayamdost, M. R. 2022, Submitted.

115. Seyedsayamdost, M. R.; Covington, B. C.; Zhang, Y.; Li, Y. 2022, In Press.

114. Bushin, L. B.; Covington, B. C.; Clark, K. A.; Caruso, A.; Seyedsayamdost, M. R. 2022, In Press.

113. Kayrouz, C. M.; Seyedsayamdost, M. R. A Novel and Prevalent Pathway in Microbial Selenium Metabolism. BioRxiv 2022, DOI: 10.1101/2022.04.13.486033 .

112. Lee, S. R.; Seyedsayamdost, M. R. Induction of Diverse Cryptic Fungal Metabolites by Steroids and Channel Blockers. Angew. Chem. Int. Ed. 2022, In Press.

111. Zhang, Y.; Gallant, É.; Park, J.-D.; Seyedsayamdost, M. R. Annu. Rev. Microbiol. 2022, In Press.

110. Covington, B. C.; Seyedsayamdost, M. R. Methods Enzymol. 2022, In Press.

109. Clark, K. A.; Bushin, L. B.; Seyedsayamdost, M. R. RaS-RiPPs in Streptococci and the Human Microbiome. ACS Bio. Med. Chem. Au 2022,  In Press.

108. Horwitz, S. M.; Blue, T. C.; Ambarian, J. A.; Hoshino, S.; Seyedsayamdost, M. R., Davis, K. M.  In Press. Structural Insights into Inhibition of the Drug Target Dihydroorotate Dehydrogenase by Bacterial Hydroxyalkylquinolines. RSC Chem. Biol. 2022, In Press.

107. Primdahl, K. G.; Kaupang, Å.; Park, J.-D.; Seyedsayamdost, M. R. ; Nolsøe, J. M. J.; Aursnes, M. On the Structure of Thailandene A: Synthetic Examination of the Cryptic Natural Product Aided by a Theoretical Approach. Synthesis 2022, 54, 1733-1744.

106. Park, J.-D.*; Li, Y.*; Moon, K.*; Han, E. J.; Lee, S. R.; Seyedsayamdost, M. R. Structural Elucidation of Cryptic Algaecides in Marine Algal-Bacterial Symbioses by NMR Spectroscopy and MicroED. Angew. Chem. Int. Ed. 2022, 61, e202114022.

105. Clark, K. A.; Covington, B. C.; Seyedsayamdost, M. R. Biosynthesis-guided Discovery of Enteropeptins, Unusual Sactipeptides Containing an N-Methylornithine. ChemRxiv 2021, DOI: 10.26434/chemrxiv-2021-jnv18.

104. Covington, B. C.; Seyedsayamdost, M. R. MetEx, a Metabolomics Explorer Application for Natural Product Discovery. ACS Chem. Biol. 2021, 16, In Press.

103. Wang, R.; Gallant, É.; Wilson, M. Z.; Wu, Y.; Li, A.; Gitai, Z.; Seyedsayamdost, M. R. Algal p-Coumaric Acid Induces Oxidative Stress and Siderophore Biosynthesis in the Bacterial Symbiont Phaeobacter inhibens. Cell Chem. Biol. 2021, 29, In Press.

102. Caruso, A.; Seyedsayamdost, M. R. Radical SAM Enzyme QmpB Installs Two 9-Membered Ring Macrocycles During Biogenesis of a Ribosomal Peptide Natural Product. J. Org. Chem. 2021, 86, 11284-11289.

101. Chioti, V. T.; McWhorter, K. L.; Xu, F.; Jeffrey, P. D.; Davis, K. M.; Seyedsayamdost, M. R. Structural and Functional Analysis of Keratinicyclin Reveals Synergistic Antibiosis with Vancomycin Against Clostridium difficile. ChemRxiv 2021, DOI: 10.26434/chemrxiv.14668545.v1.

100. Li, A.; Okada, B. K.; Rosen, P. C.; Seyedsayamdost, M. R. Piperacillin Triggers Virulence Factor Biosynthesis via the Oxidative Stress Response in Burkholderia thailandensis. Proc. Natl. Acad. Sci. USA 2021, 118, e2021483118.

99. Balo, A. R.; Caruso, A.; Tao, L.; Tantillo, D. J.; Seyedsayamdost, M. R.; Britt, R. D. Trapping a Cross-linked Lysine-Tryptophan Radical in the Catalytic Cycle of the Radical SAM Enzyme SuiB. Proc. Natl. Acad. Sci. USA 2021, 118, e2101571118.

98. Covington, B. C.; Xu, F.; Seyedsayamdost, M. R. A Natural Product Chemist’s Guide to Unlocking Silent Biosynthetic Gene Clusters. Annu. Rev. Biochem. 2021, 90, 763-788.

97. Rued, B. E.; Covington, B. C.; Bushin, L. B.; Szewczyk, G.; Laczkovich, I.; Seyedsayamdost, M. R.; Federle, M. J. Quorum Sensing in Streptococcus mutans Regulates Production of a Novel Class of Antimicrobial Compounds. mBio, 2021, 12, e02688-20.

96. Chen, Y.-C.; Seyedsayamdost, M. R.; Ringstad, N. A Microbial Metabolite Synergizes with Serotonin to Trigger C. elegans Reproductive Behavior. Proc. Natl. Acad. Sci. USA 2020, 117, 30589-30598.

95. Klaus, J. R.; Coulon, P. M. L.; Koirala, P.; Seyedsayamdost, M. R.; Déziel, E.; Chandler, J. R. Secondary Metabolites from the Burkholderia pseudomallei Complex: Structure, Ecology, and Evolution. J. Ind. Microbiol. Biotechnol. 2020, 47, 877-887.

94. Vrla, G. D.; Esposito, M.; Zhang, C.; Kang, Y.; Seyedsayamdost, M. R.; Gitai, Z. Cytotoxic Alkyl-quinolones Mediate Surface-induced Virulence in Pseudomonas aeruginosa. PLoS Pathog. 2020, 16, e1008867.

93. Bushin, L. B.; Covington, B. C.; Rued, B. E.; Federle, M. J.; Seyedsayamdost, M. R. Discovery and Biosynthesis of Streptosactin, a Sactipeptide with an Alternative Topology Encoded by Commensal Bacteria in the Human Microbiome. J. Am. Chem. Soc. 2020, 142, 16265-16275.

92. Yoshimura, A.; Covington, B. C.; Gallant, É.; Zhang, C.; Li, A.; Seyedsayamdost, M. R. Unlocking Cryptic Metabolites with Mass-Spectrometry-Guided Transposon Mutant Selection. ACS Chem. Biol. 2020, 15, 2766-2774.

91. Zhang, C. Seyedsayamdost, M. R. Discovery of a Cryptic Depsipeptide from Streptomyces ghanaensis via MALDI-MS-Guided High-Throughput Elicitor Screening. Angew. Chem. Int. Ed. 2020, 59, 23005-23009.

90. Forneris, C.C.*; Nguy, A. K. L.*; Seyedsayamdost, M. R. Mapping and Exploiting the Promiscuity of OxyB toward Biocatalytic Production of Vancomycin Aglycone Variants. ACS Catal. 2020, 10, 9287–9298.

89. Zhang, C.; Seyedsayamdost, M. R. CanE, an Iron/2-Oxoglutarate-Dependent Lasso Peptide Hydroxylase from Streptomyces canus. ACS Chem. Biol. 2020, 15, 890–894.

88. Seyedsayamdost, M. R.; Stallforth, P. Special Issue in Honor of Professor Jon Clardy. J. Nat. Prod. 2020, 83, 565–568.

87. Greenberg, E. P.; Chandler, J. R.; Seyedsayamdost, M. R. The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy. J. Nat. Prod. 2020, 83, 738–743.

86. Mao, D.*; Yoshimura, A.*; Wang, R.; Seyedsayamdost, M. R. Reporter-Guided Transposon Mutant Selection for Activation of Silent Biosynthetic Gene Clusters in Burkholderia thailandensis. ChemBioChem 2020, 21, 1826–1831.

85. Li, A.*; Mao, D.*; Yoshimura, A.*; Rosen, P. C.; Martin, W. L.; Gallant, É.; Wühr, M.; Seyedsayamdost, M. R. Multi-Omic Analyses Provide Links between Low-Dose Antibiotic Treatment and Induction of Secondary Metabolism in Burkholderia thailandensis. mBio 2020, 11, e03210-19.

84. Park, J.-D.*; Moon, K.*; Miller, C.; Rose, J.; Xu, F.; Ebmeier, C. C.; Jacobsen, J. R.; Mao, D.; Old, W. M.; DeShazer, D.; Seyedsayamdost, M. R. Thailandenes, Cryptic Polyene Natural Products Isolated from Burkholderia thailandensis Using Phenotype-Guided Transposon Mutagenesis. ACS Chem. Biol. 2020, 15, 1195–1203.

83. Gallant, É.; Li, A.; Davis, K. M.; Seyedsayamdost, M. R. Burkholderia-Derived Natural Products: From Discovery to Target Identification toward Chemical Ecology. Invited Book Chapter Submission: “Comprehensive Natural Products III” Edited by Tadhg Begley and Ben Liu. Elsevier, pg. 121–141 (2020).

82. Seyedsayamdost, M. R.; Caruso, A.; Davis, K. M. The Chemistry and Structural Enzymology of RiPP-Modifying Radical SAM Metalloenzymes. Invited Book Chapter Submission: “Comprehensive Natural Products III” Edited by Tadhg Begley and Ben Liu. Elsevier, pg. 49–64 (2020).

81. Isley, N. A.; Endo, Y.; Wu, Z. C.; Covington, B. C.; Bushin, L. B.; Seyedsayamdost, M. R.; Boger, D. L. Total Synthesis and Stereochemical Assignment of Streptide. J. Am. Chem. Soc. 2019, 141, 17361–17369.

80. Caruso, A.; Martinie, R. J.; Bushin, L. B.; Seyedsayamdost, M. R. Macrocyclization via an Arginine-Tyrosine Crosslink Broadens the Reaction Scope of Radical SAM Enzymes. J. Am. Chem. Soc. 2019, 141, 16610–16614.

79. Clark, K. A.; Bushin, L. B.; Seyedsayamdost, M. R. Aliphatic Ether Bond Formation Expands the Scope of Radical SAM Enzymes in Natural Product Biosynthesis.  J. Am. Chem. Soc. 2019, 141, 10610–10615.

78. Okada, B. K.; Li, A.; Seyedsayamdost, M. R. Identification of the Hypertension Drug Guanfacine as an Anti-virulence Agent in Pseudomonas aeruginosa. ChemBioChem. 2019, 20, 2005–2011.

• Invited Submission for the “ChemBioTalents” Special Issue
• Selected as a VIP (Very Important Paper)

77. Moon, K.; Xu, F.; Seyedsayamdost, M. R. Cebulantin, A Cryptic Lanthipeptide Antibiotic Uncovered via Bioactivity-Coupled HiTES. Angew. Chem. Int. Ed. 2019, 58, 5973–5977.

76. Moon, K.; Xu, F.; Zhang, C.; Seyedsayamdost, M. R. Bioactivity-HiTES Unveils Cryptic Antibiotics Encoded in Actinomycete Bacteria. ACS Chem. Biol. 2019, 14, 767–774.

75. Seyedsayamdost, M. R. Toward a Global Picture of Bacterial Secondary Metabolism. J. Ind. Microbiol. Biotechnol. 2019, 46, 301–311.

74. Stubbe, J.; Seyedsayamdost, M. R. Discovery of a New Class I Ribonucleotide Reductase with an Essential DOPA Radical and NO Metal as an Initiator of Long-Range Radical Transfer. Biochemistry 2019, 58, 435–437.

73. Xu, F.; Wu, Y.; Zhang, C.; Davis, K. M.; Moon, K.; Bushin, L. B.; Seyedsayamdost, M. R. A Genetics-Free Method for High-Throughput Discovery of Cryptic Microbial Metabolites. Nat. Chem. Biol. 2019, 15, 161–168.

72.  Caruso, A.; Bushin, L. B.; Clark, K. A.; Martinie, R. J.; Seyedsayamdost, M. R. A Radical Approach to Enzymatic β-Thioether Bond Formation. J. Am. Chem. Soc. 2019, 141, 990–997.

71. Bushin, L. B.; Clark, K. A.; Pelczer, I.; Seyedsayamdost, M. R. Charting an Unexplored Streptococcal Biosynthetic Landscape Reveals a Unique Peptide Cyclization Motif. J. Am. Chem. Soc. 2018, 140, 17674–17684.

70. McRose, D. L.; Seyedsayamdost, M. R.; Morel F. M. M. Multiple Siderophores: Bug or Feature? J. Biol. Inorg. Chem. 2018, 23, 983-993.

69. Wu, Y.; Seyedsayamdost, M. R. The Polyene Natural Product Thailandamide A Inhibits Fatty Acid Biosynthesis in Gram-Positive and Gram-Negative Bacteria. Biochemistry 2018, 57, 4247-4251.

68. McRose, D. L.; Baars, O.; Seyedsayamdost, M. R.; Morel, F. M. M. Quorum Sensing and Iron Regulate a Two-for-One Siderophore Gene Cluster in Vibrio harveyi. Proc. Natl. Acad. Sci. USA. 2018, 115, 7581-7586.

67. Mao, D.*; Okada, B. K.*; Wu, Y.*; Xu, F.; Seyedsayamdost, M. R. Recent Advances in Activating Silent Biosynthetic Gene Clusters in Bacteria. Curr. Opin. Microbiol. 2018, 45, 156-163.

• Invited Submission for the “Antimicrobials” Special Issue edited by Profs. G. van Wezel & G. Wright.

66. Ozturk, S.; Forneris, C. C.; Nguy, A. K. L.; Sorensen, E. J.; Seyedsayamdost, M. R. Modulating OxyB-Catalyzed Cross-Coupling Reactions in Vancomycin Biosynthesis by Incorporation of Diverse D-Tyr Analogs. J. Org. Chem. 2018, 83, 7309-7317.

• Invited Submission for the “Synthesis of Antibiotics & Related Molecules” Special Issue edited by Profs. W. van der Donk, M. Movassaghi & K. Tatsuta.

65. Bushin, L. B.; Seyedsayamdost, M. R. Guidelines for Determining the Structures of Radical SAM Enzyme-Catalyzed Modifications in the Biosynthesis of RiPP Natural Products. Methods Enzymol. 2018, 606, 439–460.

• Invited Submission for the “Radical SAM Enzymes” Special Issue edited by Prof. V. Bandarian.

64. Klaus, J. R.; Deay, J.; Neuenswander, B.; Hursh, W.; Gao, Z.; Bouddhara, T.; Williams, T. D.; Douglas, J.; Monize, K.; Martins, P.; Majerczyk, C.; Seyedsayamdost, M. R.; Peterson, B. R.; Rivera, M.; Chandler, J. R. Malleilactone is a Burkholderia pseudomallei virulence factor regulated by antibiotics and quorum sensing. J. Bacteriol. 2018, 14, 1-15.

63. Forneris, C. C.; Seyedsayamdost, M. R. In Vitro Reconstitution of OxyC Activity Enables Total Chemo-Enzymatic Syntheses of Vancomycin Aglycone Variants. Angew. Chem. Int. Ed. 2018, 57, 8048-8052.

62. Forneris, C. C.*; Ozturk, S.*; Sorensen, E. J.; Seyedsayamdost, M. R. Installation of Multiple Aryl Ether Crosslinks onto Non-native Substrate Peptides by the Vancomycin OxyB. Tetrahedron 2018, 74, 3231-3237.  

• Invited Submission for Prof. Herzon’s Tetrahedron Young Investigator Award Special Issue.

61. Gibson, M. I.; Seyedsayamdost, M. R. Small Biomolecules for Big Applications. ACS Cent. Sci. 2018, 4, 437-439.

60. Schramma, K. R.; Forneris, C. C.; Caruso, A.; Seyedsayamdost, M. R. Mechanistic Investigations of Lysine-Tryptophan Crosslink Formation Catalyzed by Streptococcal Radical SAM Enzymes. Biochemistry 2018, 57, 461-468.

59. Baars, O.; Zhang, X.; Gibson, M. I.; Stone, A. T.; Morel, F. M. M.; Seyedsayamdost, M. R. Crochelins, Siderophores with a Unprecedented Iron-Chelating Moiety from the Nitrogen-Fixing Bacterium Azotobacter chroococcum. Angew. Chem. Int. Ed. 2018, 57, 536-541.

58. Wu, Y.; Seyedsayamdost, M. R. Synergy and Target Promiscuity Drive Evolutionary Divergence in Bacterial Alkylquinolone Biosynthesis. Cell Chem. Biol. 2017, 24, 1437-1444.

57. Wang, R.; Seyedsayamdost, M. R. (2017) Roseochelin B, an Algaecidal Natural Product Synthesized by the Roseobacter Phaeobacter inhibens in Response to Algal Sinapic Acid. Org. Lett. 19, 5138-5141.

56. Gibson, M. I.; Forneris, C. C.; Seyedsayamdost, M. R. Peptide Crosslinks Catalyzed by Metalloenzymes in Natural Products Biosynthesis. Invited Book Chapter Submission: “Cyclic Peptides” Edited by Jesko Koehnke, James Naismith, Wilfred A. van der Donk. RSC Press, pg. 141–163 (2017).

55. Rosen, P. C.; Seyedsayamdost, M. R. Though Much is Taken, Much Abides: Finding New Antibiotics Using Old Ones. Biochemistry 2017, 56, 4925-4926.

54. Davis, K. M.; Schramma, K. R.; Hansen, W. A.; Bacik, J. P.; Khare, S. D.; Seyedsayamdost, M. R.; Ando, N. Structures of the Peptide-Modifying Radical SAM Enzyme SuiB Elucidate the Basis of Substrate Recognition. Proc. Natl. Acad. Sci. USA 2017, 114, 10420-10425.

53. Forneris, C. C.; Ozturk, S.; Gibson, M. I.; Sorensen, E. J.; Seyedsayamdost, M. R. In Vitro Reconstitution of OxyA Enzymatic Activity Clarifies Late Steps in Vancomycin Biosynthesis. ACS Chem. Biol. 2017, 12, 2248-2253.

52. Xu, F.; Nazari, B.*; Moon, K.*; Bushin, L. B.; Seyedsayamdost, M. R. Discovery of a Cryptic Antifungal Compound from Streptomyces albus J1074 Using High-Throughput Elicitor Screens. J. Am. Chem. Soc. 2017, 139, 9203-9212.Highlighted in:
• “Cryptic Natural Products Appearing” (In the Pipeline, July 3rd, 2017)

51. Reece, S.Y.; Seyedsayamdost, M. R. Long-Range Proton-Coupled Electron Transfer in the Escherichia coli Class 1a Ribonucleotide Reductase. Essays Biochem. 2017, 61, 281-292.

50. Mao, D.; Bushin, L. B.; Moon, K.; Wu, Y.; Seyedsayamdost, M. R. Discovery of scmR as a Global Regulator of Secondary Metabolism and Virulence in Burkholderia thailandensis E264. Proc. Natl. Acad. Sci. USA 2017, 114, E2920-E2928.Highlighted in:
• “Scientists discover master switch to turn on silent biosynthetic gene clusters” (Drug Discovery and Development, April 24, 2017)
• “Researchers switch on silent gene clusters in bacteria” (Rocket News, April 16, 2017)
• “Mini-factories for drugs? Researchers find a way to turn on silent gene clusters in bacteria” (International Business Times, April 16, 2017)
• “Researchers uncover global regulator that switches on silent biosynthetic gene clusters” (The Medical News, April 13, 2017)
• “Scientists discover master switch to turn on silent biosynthetic gene clusters” (Home Biology Cell, April 13, 2017)
• “Scientists discover master switch to turn on silent biosynthetic gene clusters” (EurekAlert!, April 13, 2017)

49. Schramma, K. R.; Seyedsayamdost, M. R. Lysine-Tryptophan-Crosslinked Peptides Produced by Radical SAM Enzymes in Pathogenic Streptococci. ACS Chem. Biol. 2017, 12, 922-927.

48. Wang, R.; Seyedsayamdost, M. R. Hijacking Exogenous Signals to Generate New Secondary Metabolites in Symbiotic Interactions. Nat. Rev. Chem. 2017, 1, 21.

Highlighted on the Cover of Nature Reviews Chemistry, March Issue, 2017:

47. Nazari, B.; Forneris, C. C.; Gibson, M. I.; Moon, K.; Schramma, K. R.; Seyedsayamdost, M. R. Nonomuraea sp. ATCC 55076 Harbours the Largest Actinomycete Chromosome to Date and the Kistamicin Biosynthetic Gene Cluster. Med. Chem. Commun. 2017, 8, 780-788.

• Invited Submission for “New Talent: Americas” Special Issue.

46. Papenfort, K.; Silpe, J. E.; Schramma, K. R.; Cong, J.-P.; Seyedsayamdost, M. R.; Bassler, B. L. A Vibrio cholerae Autoinducer-Receptor Pair that Controls Biofilm Formation. Nat. Chem. Biol. 2017, 13, 551-557.Highlighted in:
• “Microbiology: A new language for small talk” (Nat. Chem. Biol. 2017, 13, 453-454)

45. Seyedsayamdost, M. R. Talking with Molecules: Marine Bacteria and Microalgae. Invited Book Chapter Submission: “The Chemistry of Microbimes”  Edited by Tina Bahadori, Carole Bewley, Edward DeLong, Jim Fredrickson, Barabara Gerratana, David Rockcliffe. The National Academies Press, pg. 77–84 (2017).

44. Metelev, M.; Arseniev, A.; Bushin, L. B.; Kuznedelov, K.; Artamonova, T. O.; Kondratenko, R.; Khodorkovskii, M.; Seyedsayamdost, M. R.; Severinov, K. Acinetodin and Klebsidin, RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae. ACS Chem. Biol. 2017, 12, 814-824.

43. Okada, B. K.; Seyedsayamdost, M. R. Antibiotic Dialogues: Induction of Silent Biosynthetic Gene Clusters by Exogenous Small Molecules. FEMS Microbiol. Rev. 2017, 41, 19–33.

Highlighted in:
• “Discovering Novel Antibiotics” (The Scientist Magazine, February 1, 2017)

42. Okada, B. K.*; Wu, Y.*; Mao, D.; Bushin, L. B.; Seyedsayamdost, M. R. Mapping the Trimethoprim-Induced Secondary Metabolome of Burkholderia thailandensis. ACS Chem. Biol. 2016, 11, 2124–2130.Highlighted in:
• F1000 Prime (by David Newman, May 11, 2017)

41. Wang, R.; Gallant, É.; Seyedsayamdost, M. R. Investigation of the Genetics and Biochemistry of Roseobacticide Production in the Roseobacter Clade Bacterium Phaeobacter inhibens. mBio 2016, 7, e02118.

40. Wilson, M. Z.; Wang, R.; Gitai, Z.; Seyedsayamdost, M. R.  Mode of Action and Resistance Studies Unveil New Roles for Tropodithietic Acid as an Anticancer Agent and the γ-Glutamyl Cycle as a Proton Sink.  Proc. Natl. Acad. Sci. USA 2016, 113, 1630-1635.

Highlighted in:
• “Antibiotics that Fight Cancer” (BioTechniques, March 3, 2016)

39. Baars, O.; Zhang, X.; Morel, F. M.; Seyedsayamdost, M. R.  The Siderophore Metabolome of Azotobacter vinelandii.  Appl. Environ. Microbiol. 2015, 82, 27-39.

38. Truong, T. T.; Seyedsayamdost. M. R.; Greenberg, E. P.; Chandler, J. R.  A Burkholderia thailandensis Acyl-Homoserine Lactone-Independent Orphan LuxR Homolog that Activates Production of the Cytotoxin Malleilactone.  J. Bacteriol. 2015, 197, 3456-3462.Highlighted in:
• “Antibiotic Sensing by a Bacterial Communication System” (Atlas of Science, December 22, 2015)

37. Schramma, K. R.*; Bushin, L. B.*; Seyedsayamdost, M. R.  Structure and Biosynthesis of a Macrocyclic Peptide Containing an Unprecedented Lysine-to-Tryptophan Crosslink.  Nature Chem. 2015, 7, 431-437.

Highlighted in:
• “News of the Week” (C&E News 2015, 93, 8)
• “Spotlight” (ACS Chem. Biol. 2015, 10, 1355)
• “Cellular Microbiology” (Scicasts, May 18, 2015)

36. Seyedsayamdost, M. R.*; Wang, R.; Kolter, R.; Clardy, J.* Hybrid Biosynthesis of Roseobacticides from Algal and Bacterial Precursor Molecules.  J. Am. Chem. Soc. 2014, 136, 15150-15153.

35. Seyedsayamdost, M. R.  High-Throughput Platform for the Discovery of Elicitors of Silent Bacterial Gene Clusters.  Proc. Natl. Acad. Sci. USA 2014, 111, 7266-7271.

Highlighted in:
• “This Week In PNAS” (Proc. Natl. Acad. Sci. USA 2014, 111, 7163)
• “Spotlight” (ACS Chem. Biol. 2014, 9, 1218)
• “GEN News Highlight” (Genetic Engineering & Biotechnology News, May 21, 2014)

Prior Publications

34. Seyedsayamdost, M. R.; Clardy J.  Natural Products and Synthetic Biology.  ACS Synth. Biol. 2014, 3, 745-747.

33. Wörsdörfer, B. A.; Conner, D. A.; Yokoyama, K.; Livada, J.; Seyedsayamdost, M. R.; Jiang, W.; Silakov, A.; Stubbe, J.; Bollinger, J. M. Jr.; Krebs, C.  J.  Function of the diiron cluster of the Escherichia coli class Ia ribonucleotide reductase in proton-coupled electron transfer.  J. Am. Chem. Soc. 2013, 135, 8585-8593.

32. Chandler, J. R.; Truong, T. T.; Silva, P. M.; Seyedsayamdost, M. R.; Carr, G.; Radey, M.; Jacobs, M. A.; Sims, E. H.; Clardy, J.; Greenberg, E. P. Bactobolin resistance is conferred by mutations in the L2 ribosomal protein.  MBio 2012, 3, e0049-e00512.

31. Seyedsayamdost, M. R.; Traxler, M. F.; Clardy, J.; Kolter, R.  Old meets new: using interspecies interactions to detect secondary metabolite production in actinomycetes.  Methods Enzymol. 2012, 517, 89-109.

30. Adler, C.; Corbalan, N. S.; Seyedsayamdost, M. R.; Pomares, M. F.; de Cristobal, R. E.; Clardy, J.; Kolter, R.; Vincent, P. A.  Catecholate siderophores protect bacteria from pyochelin toxicity.  PloS one 2012, 7, e46754-e46760.

29. Traxler, M. F.; Seyedsayamdost, M. R.; Clardy, J.; Kolter, R.  Interspecies modulation of bacterial development through iron competition and siderophore piracy.  Mol. Microbiol. 2012, 86, 628-644.

28. Seyedsayamdost, M. R.; Cleto, S.; Carr, G.; Vlamakis, H.; Vieira, M. J.; Kolter, R.; Clardy, J.  Mixing and Matching Siderophore Clusters: Structure and Biosynthesis of Serratiochelins from Serratia sp. V4.  J. Am. Chem. Soc. 2012, 134, 13550-13553.

27. Fernandes, N.; Case, R. J.; Longford, S. R.; Seyedsayamdost, M. R.; Steinberg, P. D.; Kjelleberg, S.; Thomas, T.  Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra.  PloS one 2011, 6, e27387-e27398.

26. Seyedsayamdost, M. R.; Carr, G.; Kolter, R.; Clardy, J.  Roseobacticides: Small Molecule Modulators of an Algal-Bacterial Symbiosis.  J. Am. Chem. Soc. 2011, 133, 18343-18349.

25 Seyedsayamdost, M. R.; Traxler, M. F.; Zheng, S.-L.; Kolter, R.; Clardy, J.  Structure and Biosynthesis of Amychelin, an Unusual Mixed-Ligand Siderophore from Amycolatopsis sp. AA4.  J. Am. Chem. Soc. 2011, 133, 11434-11437.

24. Carr, G.; Seyedsayamdost, M. R.; Chandler, J. R.; Greenberg, E. P.; Clardy, J.  Sources of Diversity in Bactobolin Biosynthesis by Burkholderia thailandensis E264. Org. Lett. 2011, 13, 3048-3051.

23. Seyedsayamdost, M. R.; Case, R. J.; Kolter, R.; Clardy, J.  The Jekyll-and-Hyde chemistry of Phaeobacter gallaeciensis.  Nature Chem. 2011, 3, 331-335.

22. Seyedsayamdost, M. R.; Yee, C. S.; Stubbe, J.  Use of 2,3,5-F3Y-ß2 and 3-NH2Y-α2 to Study Proton-Coupled Electron Transfer in Escherichia coli Ribonucleotide Reductase.  Biochemistry 2011, 50, 1403-1411.

21. Minnihan, E. C.; Seyedsayamdost, M. R.; Uhlin, U.; Stubbe, J.  Kinetics of Radical Intermediate Formation and Deoxynucleotide Production in 3-Aminotyrosine-Substituted Escherichia coli Ribonucleotide Reductases. J. Am. Chem. Soc. 2011, 133, 9430-9440.

20. Seyedsayamdost, M. R.; Chandler, J. R.; Blodgett, J. A. V.; Lima, P. S.; Duerkop, B. A.; Oinuma, K.-I.; Greenberg, E. P.; Clardy, J., Quorum-Sensing-Regulated Bactobolin Production by Burkholderia thailandensis E264. Org. Lett. 2010, 12, 716-719.

19. Seyedsayamdost, M. R.; Stubbe, J.  Replacement of Y730 and Y731 in the α2 Subunit of Escherichia coli Ribonucleotide Reductase with 3-Aminotyrosine Using an Evolved Suppressor tRNA/tRNA Synthetase Pair.  Methods Enzymol. 2009; 462, 45-76.

18. Seyedsayamdost, M. R.; Argirevic, T.; Minnihan, E. C.; Stubbe, J.; Bennati, M.  Structural Examination of the Transient 3-Aminotyrosyl Radical on the PCET Pathway of E. coli Ribonucleotide Reductase by Multifrequency EPR Spectroscopy.  J. Am. Chem. Soc. 2009, 131, 15729-15738.

17. Reece, S. Y.; Lutterman, D. A.; Seyedsayamdost, M. R.; Stubbe, J.; Nocera, D. G.  Re(bpy)(CO)(3)CN as a Probe of Conformational Flexibility in a Photochemical Ribonucleotide Reductase.  Biochemistry 2009, 48, 5832-5838.

16. Offenbacher, A. R.; Vassiliev, I. R.; Seyedsayamdost, M. R.; Stubbe, J.; Barry, B. A.  Redox-Linked Structural Changes in Ribonucleotide Reductase.  J. Am. Chem. Soc. 2009, 131, 7496-7497.

15. Minnihan, E. C.; Seyedsayamdost, M. R.; Stubbe, J.  Use of 3-Aminotyrosine To Examine the Pathway Dependence of Radical Propagation in Escherichia coli Ribonucleotide Reductase.  Biochemistry 2009, 48, 12125-12132.

14. Seyedsayamdost, M. R.; Yee, C. S.; Stubbe, J.  Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation.  Nat. Protoc. 2007, 2, 1225-1235.

13. Seyedsayamdost, M. R.; Chan, C. T. Y.; Mugnaini, V.; Stubbe, J.; Bennati, M.  PELDOR spectroscopy with DOPA-beta 2 and NH2Y-alpha 2s: Distance measurements between residues involved in the radical propagation pathway of E-coli ribonucleotide reductase.  J. Am. Chem. Soc. 2007, 129, 15748-15749.

12. Seyedsayamdost, M. R.; Xie, J.; Chan, C. T. Y.; Schultz, P. G.; Stubbe, J.  Site-specific insertion of 3-aminotyrosine into subunit α2 of E. coli ribonucleotide reductase: Direct evidence for involvement of Y730 and Y731 in radical propagation.  J. Am. Chem. Soc. 2007, 129, 15060-15071.

11. Reece, S. Y.; Seyedsayamdost, M. R.; Stubbe, J.; Nocera, D. G. Direct observation of a transient tyrosine radical competent for initiating turnover in a photochemical ribonucleotide reductase.  J. Am. Chem. Soc. 2007, 129, 13828-13830.

10. Reece, S. Y.; Seyedsayamdost, M. R.; Stubbe, J.; Nocera, D. G.  Photoactive peptides for light-initiated tyrosyl radical generation and transport into ribonucleotide reductase.  J. Am. Chem. Soc. 2007, 129, 8500-8509.

9. Seyedsayamdost, M. R.; Stubbe, J.  Forward and reverse electron transfer with the Y356DOPA-β2 heterodimer of E. coli ribonucleotide reductase. J. Am. Chem. Soc. 2007, 129, 2226-2227.

8. Reece, S. Y.; Seyedsayamdost, M. R.; Stubbe, J.; Nocera, D. G.  Electron transfer reactions of fluorotyrosyl radicals.  J. Am. Chem. Soc. 2006, 128, 13654-13655.

7. Seyedsayamdost, M. R.; Stubbe, J.  Site-specific replacement of Y356 with 3,4-dihydroxyphenylalanine in the β2 subunit of E. coli ribonucleotide reductase.  J. Am. Chem. Soc. 2006, 128, 2522-2523.

6. Seyedsayamdost, M. R.; Reece, S. Y.; Nocera, D. G.; Stubbe, J.  Mono-, di-, tri-, and tetra-substituted fluorotyrosines: New probes for enzymes that use tyrosyl radicals in catalysis.  J. Am. Chem. Soc. 2006, 128, 1569-1579.

5. Seyedsayamdost, M. R.; Yee, C. S.; Reece, S. Y.; Nocera, D. G.; Stubbe, J.  pH rate profiles of FnY356-R2s (n=2, 3, 4) in Escherichia coli ribonucleotide reductase: Evidence that Y-356 is a redox-active amino acid along the radical propagation pathway.  J. Am. Chem. Soc. 2006, 128, 1562-1568.

4. Guillen Schlippe, Y. V.; Riera, T. V.; Seyedsayamdost, M. R.; Hedstrom, L.  Substitution of the conserved Arg-Tyr dyad selectively disrupts the hydrolysis phase of the IMP dehydrogenase reaction.  Biochemistry 2004, 43, 4511-21.

3. Yee, C. S.; Seyedsayamdost, M. R.; Chang, M. C. Y.; Nocera, D. G.; Stubbe, J., Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: Insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry 2003, 42, 14541-14552.

2. Hedstrom, L.; Gan, L.; Schlippe, Y. G.; Riera, T.; Seyedsayamdost, M.  IMP dehydrogenase: the dynamics of drug selectivity. Nucleic Acids Res. Supp. 2003, 3, 97-8.

1. Gan, L.; Seyedsayamdost, M. R.; Shuto, S.; Matsuda, A.; Petsko, G. A.; Hedstrom, L.  The immunosuppressive agent mizoribine monophosphate forms a transition state analogue complex with inosine monophosphate dehydrogenase.  Biochemistry 2003, 42, 857-863.